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Image Search Results
Journal: Cancer research
Article Title: Hedgehog signaling regulates metabolism and polarization of mammary tumor-associated macrophages
doi: 10.1158/0008-5472.CAN-20-1723
Figure Lengend Snippet: A. Schematic of O-GlcNAcylation. Ogt adds O-GlcNAc to target proteins from the substrate UDP-GlcNAc, and Oga removes O-GlcNAc moieties. B. Immunoblot for all cellular O-GlcNAcylated proteins (using RL2 antibody) in M2 macrophages (BMDMs) +/− Hhi. C. Protein (Immunoblot) and transcript (qPCR) levels for Ogt in M2 macrophages (BMDMs) +/− Hhi. D. ChIP was performed in macrophages (skewed from RAW264.7 cells) for Gli1 binding to the Ogt promoter followed by qPCR with primers specific for Ogt promoter. Graph shows qPCR results with signal relative to input with beads subtracted. E. Immunoblots for cellular O-GlcNAcylated proteins (using RL2 antibody) in M2 macrophages sorted from 4T1 tumors and EMT6 tumors of mice treated with DMSO or Smo-i. F. Immunoblots for Ogt in tumor infiltrating M2 macrophages sorted from 4T1 and EMT6 tumors of mice treated with DMSO or Smo-i *P<0.05. These lysates were also used for data depicted in Figure 8C . As such the same image for β-actin loading control is used.
Article Snippet:
Techniques: Western Blot, Binding Assay, Control